1. Fill a small beaker with 150 mL of Tris Buffered Saline (TBS).
2. Add the following while stirring:
• 0.20 g BSA Sigma A-7030 (0.1% final)
• 300 uL Triton X-100 Baker X-198-05 (0.15% final)
3. Adjust pH to 7.4 to 7.5 and qs volume to 200 mL.
4. Filter into a 1 L orange-top Corning disposable bottle. Store at 4C.

MAM 11/05/97

1. Fill a 1 L beaker with 750 mL of ddH₂O.
2. Add the following while stirring:
• 7.88 g Tris HCl Sigma T 3253 (FW=157.6; 50 mM)
• 8.77 g sodium chloride (FW=58.44; 150 mM)
3. Adjust pH to 7.4 to 7.5 and qs volume to 1 L.
4. Filter into a 250 mL orange-top corning disposable bottle. Store at 4C.

MAM 11/05/97